Duck hepatitis A virus 1 (DHAV-1) is one of the most severe pathogens threatening the duck industry. Phosphatidylinositol-4 kinases (PI4Ks) are crucial for viral infection, with different viruses adopting various strategies to utilize PI4Ks. Whether PI4Ks proteins are involved in DHAV-1 infection has not been reported. In this study, we found that the phosphatidylinositol 4-kinase beta (PI4KB) protein, within the PI4Ks family, promotes the replication and translation of DHAV-1. The 2A2, 2C, 2BC, 3A, 3AB, 3D, and 3CD proteins of DHAV-1 were all found to interact and co-localize with the PI4KB protein. The 101-120 amino acid region of the 2A2 protein is the key area for its interaction with the PI4KB protein. This study reveal the essential role of the PI4KB protein in the replication and translation of DHAV-1, providing a theoretical basis for studying the life cycle of DHAV-1 and the development of prevention and control strategies against DHAV-1. The related research is titled “PI4KB is an essential host factor for duck hepatitis A virus 1 replication and translation” and was published in the International Journal of Biological Macromolecules, 10.1016/j.ijbiomac.2024.136480.

This work was supported by China Agricultural Research System (CARS-42-17), the Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System (SCCXTD-2024-18).