Duck plague virus (DPV) infection can lead to high mortality of ducks and other waterfowl, causing huge economic losses to the duck industry. At present, there is no specific drug to treat DPV infection effectively. BX795 is a kinase inhibitor that specifically inhibits DPK1 and TBK1. We evaluated the antiviral activity of BX795 against DPV using duck cells as study models. The results showed that the inhibitor had a very strong inhibitory effect on DPV infection. The antiviral effect of BX795 on duck embryo fibroblasts (DEF) lasted for at least 96 hours. Studies have found that BX795 inhibits virus infection by blocking virus cell-to-cell spread. Further studies showed that BX795 can exert its antiviral effect by disturbing the DPV US3 phosphorylation kinase activity and preventing the interaction with its substrates, which has potential application value in the treatment of DPV infection. The relevant study titled "BX795, a Kinase Inhibitor, Inhibit Duck Plague Virus Infection via Targeting US3 Kinase", On March 15, published in the journal Poultry Science, address is: https://doi.org/10.1016/j.psj.2023.102597 

Figure 7. BX795 disrupts DPV US3 kinase activity. The effect of BX795 on DPV US3 kinase activity was analyzed by Western blot.

(A) BX795 significantly inhibited substrate phosphorylation by DPV US3 kinase in a dose-dependent manner. (B) BX795 can block the interaction between US3 and UL47.