Duck hepatitis A virus

Introduction

Our research line focus on avian viral hepatitis, although the human viral hepatitis research community has a great research interest on this topic, as its strong nexus to acute hepatitis and chronical hepatitis, cirrhosis and liver cancer. However, certain similar diseases that is highly prevalent in duckling or mature ducks was not well-recognized. The mission of our study is to prevent and control epidemic of avian viral hepatitis, especially infection caused by duck hepatitis A virus (DHAV). Because infection by the DHAV is highly fatal and their prevalence in China is currently high, in particular genotype 1 and genotype 3. To do so, multidisciplinary knowledge has been integrated to better understand viral pathogenesis, viral functional proteomics (i.e., 3C), virus-host interactions upon infection by DHAV and other relevant hepatitis viruses. We hope that our research will add new mechanistical perception of avian viral hepatitis, thus may completely prevent or eliminate this disease by exploiting novel vaccine technologies, new measures or drugs from basic research. With three decades of effort, Prof. Anchun Cheng, the founder of our faculty, has successfully developed a live attenuated DHAV vaccine (genotype 1) and now widely applied in duck industry. Other bio-pharmacal agents including egg yolk immunoglobulins has been developed and show considerable clinical benefit in infected ducklings. Followed by this direction, three youth scientists were joined and continued to focus on the avian viral hepatitis. Until September 2022, we have contributed to 35 SCI-cited publications which most of them are published in TOP ranked journals in veterinary fields. These publications were funded by both "13th Five-Year" National Key Research and Development Plan project, national scientific research project of Sichuan province, and NSFC grants. Let’s do science corporately and try to vanish it.

Research interests

1. DHAV Pathogenesis
2. Classical and bystander functions of DHAV proteins (3C protease) and its role in viral replication
3. Host factors that interact with DHAV proteins
4. Host immune defense: inflammasome, defensin, and cell death
5. tRNA biology, viral hepatitis and liver cancer

Selected publications

1. Mao, Sai, Mingshu Wang, Xumin Ou, Di Sun, Anchun Cheng, Dekang Zhu, Shun Chen et al. "Virologic and immunologic characteristics in mature ducks with acute duck hepatitis A virus 1 infection." Frontiers in immunology 8 (2017): 1574.
2. Mao, Sai, Xumin Ou, Mingshu Wang, Di Sun, Qiao Yang, Ying Wu, Renyong Jia et al. "Duck hepatitis A virus 1 has lymphoid tissue tropism altering the organic immune responses of mature ducks." Transboundary and Emerging Diseases 68, no. 6 (2021): 3588-3600.
3. Mao, Sai, XuMin Ou, DeKang Zhu, Shun Chen, GuangPeng Ma, MingShu Wang, RenYong Jia et al. "Development and evaluation of indirect ELISAs for the detection of IgG, IgM and IgA1 against duck hepatitis A virus 1." Journal of virological methods 237 (2016): 79-85.
4. Sun, Di, Shun Chen, Anchun Cheng, and Mingshu Wang. "Roles of the picornaviral 3C proteinase in the viral life cycle and host cells." Viruses 8, no. 3 (2016): 82.
5. Di Sun, Mingshu Wang, et al. Cleavage of poly(A)-binding protein by duck hepatitis A virus 3C protease. Scientific Reports, 2017, 7(1):11.
6. Di Sun, Mingshu Wang, et al. Biochemical characterization of recombinant Avihepatovirus 3C protease and its localization. Virology Journal, 2019, 16(1):13.
7. Sun, Di, Xingjian Wen, Mingshu Wang, Sai Mao, Anchun Cheng, Xiaoyao Yang, Renyong Jia et al. "Apoptosis and autophagy in picornavirus infection." Frontiers in Microbiology 10 (2019): 2032.
8. Wen, Xingjian, Jinlong Guo, Di Sun, Mingshu Wang, Dian Cao, Anchun Cheng, Dekang Zhu et al. “Mutations in VP0 and 2C proteins of duck hepatitis A virus type 3 attenuate viral infection and virulence.” Vaccines 7, no. 3 (2019): 111.
9. Wang, Jingming, Di Sun, Mingshu Wang, Anchun Cheng, Yukun Zhu, Sai Mao, Xuming Ou et al. "Multiple functions of heterogeneous nuclear ribonucleoproteins in the positive single-stranded RNA virus life cycle." Frontiers in Immunology 13 (2022).
10.Xumin Ou, Mingshu Wang, Sai Mao, Jingyu Cao, Anchun Cheng, Dekang Zhu, Shun Chen et al. "Incompatible translation drives a convergent evolution and viral attenuation during the development of live attenuated vaccine." Frontiers in cellular and infection microbiology 8 (2018): 249.
11.Ou, Xumin, Jingyu Cao, Anchun Cheng, Maikel P. Peppelenbosch, and Qiuwei Pan. "Errors in translational decoding: tRNA wobbling or misincorporation?." PLoS genetics 15, no. 3 (2019): e1008017.
12.Zhang, Ruyi, Lisanne Noordam, Xumin Ou*, Buyun Ma, Yunlong Li, Pronay Das, Shaojun Shi et al. "The biological process of lysine‐tRNA charging is therapeutically targetable in liver cancer." Liver International 41, no. 1 (2021): 206-219.
13.Xumin Ou, Buyun Ma, Ruyi Zhang, Zhijiang Miao, Anchun Cheng, Maikel P. Peppelenbosch, and Qiuwei Pan. "A simplified qPCR method revealing tRNAome remodeling upon infection by genotype 3 hepatitis E virus." FEBS letters 594, no. 12 (2020): 2005-2015.
14.Xumin Ou, Wenjing Peng, Zhishuang Yang, Jingyu Cao, Mingshu Wang, Maikel P. Peppelenbosch, Qiuwei Pan, and Anchun Cheng. "Evolutionarily missing and conserved tRNA genes in human and avian." Infection, Genetics and Evolution 85 (2020): 104460.
15.Xumin Ou, Zhishuang Yang, Dekang Zhu, Sai Mao, Mingshu Wang, Renyong Jia, Shun Chen et al. "Tracing genetic signatures of bat-to-human coronaviruses and early transmission of North American SARS-CoV-2." Transboundary and Emerging Diseases (2021).
16.Miao, Zhijiang, Shaoshi Zhang, Xumin Ou, Shan Li, Zhongren Ma, Wenshi Wang, Maikel P. Peppelenbosch, Jiaye Liu, and Qiuwei Pan. "Estimating the global prevalence, disease progression, and clinical outcome of hepatitis delta virus infection." The Journal of infectious diseases 221, no. 10 (2020): 1677-1687.
17.Ou, Xumin, Sai Mao, Jingyu Cao, Anchun Cheng, Mingshu Wang, Dekang Zhu, Shun Chen et al. "Comparative analysis of virus-host interactions caused by a virulent and an attenuated duck hepatitis A virus genotype 1." PloS one 12, no. 6 (2017): e0178993.
18.Wu, Liping, Weili Quan, Yi Zhang, Mingshu Wang, Xumin Ou, Sai Mao, Di Sun et al. "Attenuated Duck Hepatitis A Virus Infection Is Associated with High mRNA Maintenance in Duckling Liver via m6A Modification." Frontiers in immunology 13 (2022): 839677.
19.Wen, X., D. Zhu, A. Cheng, M. Wang, S. Chen, R. Jia, M. Liu et al. "Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010–2015." Transboundary and emerging diseases 65, no. 1 (2018): 10-15.

Selected invention Patents

1. Anchun Cheng, Di Sun, MingShu Wang. A kit for determining the inhibitor against 3C protease of DHAV-1. Chinese Patent No.: 201710646912 Published on: July. 06, 2021.
2. Xumin Ou, Qiuwei Pan, Anchun Cheng, Mingshu Wang, Dekang Zhu. An easy approach to detect mature tRNAome in human, Patent Reference #: ZL201811302188.X
3. Xumin Ou, Wenjing Done, Anchun Cheng, Qiuwei Pan, Mingshu Wang, Wenjing Peng, Xiaomin Lin. A qPCR-based kit for tRNA aminoacylation quantitatification. Application number: CN202210641794.4

Contact details


Xumin Ou, M.D. & Ph.D, Lecturer

Institute of Preventive Veterinary Medicine
Sichuan Agricultural University
No. 211, Huimin Road, Chengdu, Sichuan 611130, China
Email: oxumin@yahoo.com


Sai Mao, Ph.D, Lecturer

Institute of Preventive Veterinary Medicine
Sichuan Agricultural University
No. 211, Huimin Road, Chengdu, Sichuan 611130, China
Email: sarrawin@163.com


Di Sun, Ph.D, Lecturer

Institute of Preventive Veterinary Medicine
Sichuan Agricultural University
No. 211, Huimin Road, Chengdu, Sichuan 611130, China
Email: sundi9009@sicau.edu.cn