Introduction
Tembusu virus (TMUV, genus Flavivirus, family Flaviviridae) was first isolated in 1955 from Culex tritaeniorhynchus mosquitoes in Kuala Lumpur, Malaysia. In April 2010, duck TMUV was first identified as the causative agent of egg-drop syndrome, characterized by a substantial decrease in egg laying and depression, growth retardation and neurological signs or death in infected egg-laying and breeder ducks, in the China.
In 2014, the first duck strain of Tembusu virus was isolated from diseased duck in the Southwest of China (TMUV CQW1 strain, GenBank Accession: KM233707). Based on that, the monoclonal antibody against E protein, the polyclonal antibody against whole virus particle, NS1, NS2A, NS3, NS4B, and NS5 were prepared. The functional TMUV C, prM, E, NS1, NS2A, NS2B, NS3, NS4B, NS5 protein were obtained. A series of TMUV pathogen detection methods were developed including real time quantitative PCR detection of TMUV E, diagnostic kit for anti-TMUV E (Colloidal Gold), et al. Currently, duck TMUV reverse genetic systems have been constructed, including: TMUV infectious cDNA clone, reporter TMUV virus, TMUV replicons, as well as TMUV single-round infectious particle. Based on those, we are working on the following area:
1. The molecular mechanism of TMUV RNA replication.
2. The immune interplay between the host protein and duck TMUV NSs.
3. Autophagy and apoptosis induced by DTMUV infection.
4. MiRNA modulated viral replication.
5. Novel vaccine development.
Selected Publications
1. Yu He, Jiaqi Guo, Xiaoli Wang, Senzhao Zhang, Li Mao, Tao Hu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Qun Gao, Di Sun, Anchun Cheng* and Shun Chen*. Assembly-defective Tembusu virus ectopically expressing capsid protein is an approach for live-attenuated flavivirus vaccine development[J]. npj Vaccines, 2022, 7(1):51; doi:org/10.1038/s41541-022-00468-y.
2. Shanzhi Huang, Juan Huang, Min Cui, Xuedong Wu, Mingshu Wang, Dekang Zhu, Shun Chen, Mafeng Liu, Xinxin Zhao, Ying Wu, Qiao Yang, Shaqiu Zhang, Xumin Ou, Sai Mao, Qun Gao, Yanling Yu, Bin Tian, Yunya Liu, Ling Zhang, Zhongqiong Yin, Bo Jing, Xiaoyue Chen, Anchun Cheng*, Renyong Jia*. Duck Tembusu Virus Inhibits Type I Interferon Production through the JOSD1-SOCS1-IRF7 Negative-Feedback Regulation Pathway[J]. Journal of Virology, 2022, 96(18):e0093022; doi:10.1128/jvi.00930-22.
3. Tao Wang, Andres Merits, Yuanyuan Wu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Yunya Liu, Ling Zhang, Yanling Yu, Leichang Pan, Shun Chen*, Anchun Cheng*. Cis-acting Sequences and Secondary Structures in Untranslated Regions of Duck Tembusu Virus RNA Are Important for Cap-Independent Translation and Viral Proliferation[J]. Journal of Virology, 2020, 94(16):e00906-20; doi: 10.1128/JVI.00906-20.
4. Wei Zhang, Bowen Jiang, Miao Zeng, Yanping Duan, Zhen Wu, Yuanyuan Wu, Tao Wang, Mingshu Wang,Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Yunya Liu, Ling Zhang, Yanling Yu, Leichang Pan, Shun Chen*, Anchun Cheng*. Binding of Duck Tembusu virus nonstructural protein 2A to duSTING disrupts the induction of its signal transduction cascade to inhibit IFN-β induction[J]. Journal of Virology, 2020, 94(9): 01850-19; doi: 10.1128/JVI.01850-19.
5. Zhen Wu, Wei Zhang, Yuanyuan Wu, Tao Wang, Shaoxiong Wu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Yunya Liu, Ling Zhang, Yanling Yu, Leichang Pan, Shun Chen*, Anchun Cheng*. Binding of the Duck Tembusu Virus Protease to STING Is Mediated by NS2B and Is Crucial for STING Cleavage and for Impaired Induction of IFN-β[J]. Journal of Immunology, 2019, 203(12):3374-3385; doi: 10.4049/jimmunol.1900956.
6. Zhen Wu, Tao Hu, Weiqiong Chen, Yao Cheng, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Qun Gao, Di Sun, Anchun Cheng*, Shun Chen*. The autophagy-related degradation of MDA5 by Tembusu virus nonstructural 2B disrupts IFNβ production[J]. FASEB Journal, 2022, 36(7):e22417; doi: 10.1096/fj.202101916RRR.
7. Xuedong Wu, Yuetian Zhang, Mingshu Wang, Shun Chen, Mafeng Liu, Dekang Zhu, Xinxin Zhao, Ying Wu, Qiao Yang, Shaqiu Zhang, Juan Huang, Xumin Ou, Ling Zhang, Yunya Liu, Yanling Yu, Qun Gao, Sai Mao, Di Sun, Bin Tian, Zhongqiong Yin, Bo Jing, Anchun Cheng* and Renyong Jia*. Methyltransferase-Deficient Avian Flaviviruses Are Attenuated Due to Suppression of Viral RNA Translation and Induction of a Higher Innate Immunity[J]. Frontiers in Immunology, 2021, 12:751688; doi: 10.3389/fimmu.2021.751688.
8. Yu He, Xiaoli Wang, Jiaqi Guo, Li Mao, Senzhao Zhang, Tao Hu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Qun Gao, Di Sun, Yunya Liu, Ling Zhang, Yanling Yu, Anchun Cheng*, Shun Chen*. Replication/Assembly Defective Avian Flavivirus With Internal Deletions in the Capsid Can Be Used as an Approach for Living Attenuated Vaccine[J]. Frontiers in Immunology, 2021, 12:694959; doi: 10.3389/fimmu.2021.694959.
9. Wei Zhang, Miao Zeng, Bowen Jiang, Tong Lu, Jiaqi Guo, Tao Hu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Xumin Ou, Yunya Liu, Ling Zhang, Yanling Yu, Leichang Pan, Anchun Cheng*, Shun Chen*. Amelioration of Beta Interferon Inhibition by NS4B Contributes to Attenuating Tembusu Virus Virulence in Ducks[J]. Frontiers in Immunology, 2021, 12:671471; doi: 10.3389/fimmu.2021.671471.
10.Shun Chen*, Wei Zhang, Zhen Wu, Jinyue Zhang, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xiaoyue Chen and Anchun Cheng*. Goose Mx and OASL Play Vital Roles in the Antiviral Effects of Type I, II, and III Interferon against Newly Emerging Avian Flavivirus[J]. Frontiers in Immunology, 2017, 8:1006.
11.Yuhong Pan, Wenjun Cai, Anchun Cheng*, Mingshu Wang, Shun Chen, Juan Huang, Qiao Yang, Ying Wu, Di Sun, Sai Mao, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Shaqiu Zhang, Qun Gao, Xumin Ou, Bin Tian, Zhongqiong Yin and Renyong Jia*. Duck Tembusu virus infection induces mitochondrial-mediated and death receptor-mediated apoptosis in duck embryo fibroblasts[J]. Veterinary Research, 2022, 53:53; doi: org/10.1186/s13567-022-01070-9.
12.Yuhong Pan, Xuedong Wu, Wenjun Cai, Anchun Cheng*, Mingshu Wang, Shun Chen, Juan Huang, Qiao Yang, Ying Wu, Di Sun, Sai Mao, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Shaqiu Zhang, Qun Gao, Xumin Ou, Bin Tian, Zhongqiong Yin and Renyong Jia*. RNA-Seq analysis of duck embryo fibroblast cells gene expression during duck Tembusu virus infection[J]. Veterinary Research, 2022, 53:34; doi:10.1186/s13567-022-01051-y.
13.Tao Hu, Zhen Wu, Shaoxiong Wu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Qun Gao, Di Sun, Yunya Liu, Ling Zhang, YanLing Yu, Shun Chen and Anchun Cheng*. Substitutions at Loop Regions of TMUV E Protein Domain III Differentially Impair Viral Entry and Assembly[J]. Frontiers in Microbiology, 2021, 12:688172; doi: 10.3389/fmicb.2021.688172.
14.Min Cui, Shuling Chen, Shaqiu Zhang, Anchun Cheng*, Yuhong Pan, Juan Huang, Zhiqiang Hu, Xingcui Zhang, Mingshu Wang, Dekang Zhu, Shun Chen, Mafeng Liu, Xinxin Zhao, Yin Wu, Qiao Yang, Yunya Liu, Ling Zhang, Yanling Yu, Zhongqiong Yin, Bo Jing, Mujeeb Ur Rehman, Bin Tian, Leichang Pan and Renyong Jia*. Duck Tembusu Virus Utilizes miR-221-3p Expression to Facilitate Viral Replication via Targeting of Suppressor of Cytokine Signaling 5[J]. Frontiers in Microbiology, 2020, 11:596; doi: 10.3389/fmicb.2020.00596.
15.Miao Zeng, Shun Chen*, Wei Zhang, Yanping Duan, Bowen Jiang, Xin Pan, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng , Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Sai Mao, Bin Tian, Qun Gao, Anchun Cheng*. Nuclear localization of duck Tembusu virus NS5 protein attenuates viral replication in vitro and NS5-NS2B3 interaction[J].Veterinary Microbiology, 2021, 262:109239; doi: org/10.1016/j.vetmic.2021.109239.
16.Senzhao Zhang, Xiaoli Wang, Yu He, Tao Hu, Jiaqi Guo, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Qun Gao, Di Sun, Yunya Liu, Ling Zhang, Shun Chen*, Anchun Chen*. N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings[J]. Veterinary Microbiology, 2021, 261:109215; doi: 10.1016/j.vetmic.2021.109215.
17.Xingcui Zhang, Renyong Jia*, Yuhong Pan, Mingshu Wang, Shun Chen, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Yunya Liu, Ling Zhang, Zhongqiong Yin, Bo Jing, Juan Huang, Bin Tian, Leichang Pan, Yanling Yu, Mujeeb Ur Rehman, Anchun Cheng*. Therapeutic effects of duck Tembusu virus capsid protein fused with staphylococcal nuclease protein to target Tembusu infection in vitro[J]. Veterinary Microbiology, 2019(235): 295-300.
18.Juan Huang, Haoyue Shen, Renyong Jia*, Mingshu Wang, Shun Chen, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Yunya Liu, Ling Zhang, Zhongqiong Yin, Bo Jing and Anchun Cheng*. Oral Vaccination with a DNA Vaccine Encoding Capsid Protein of Duck Tembusu Virus Induces Protection Immunity[J]. Viruses, 2018, 10(4):180; doi:10.3390/v10040180.
19.Hao Zhou, Shun Chen*, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Fei Liu, Qiao Yang, Ying Wu, Kunfeng Sun, Xiaoyue Chen, Bo Jing & Anchun Cheng*. Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ[J]. Scientific Reports, 2016, 6:25545.
20.Kesen Zhu, Juan Huang, Renyong Jia*, Bin Zhang, Mingshu Wang, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Anchun Cheng*. Identification and molecular characterization of a novel duck Tembusu virus isolate from Southwest China[J]. Archives of Virology, 2015, 160(11):2781-2790.
Invention Patents
1. Shun Chen, Yu He, Anchun Cheng, Mingshu Wang. A method for construction of duck Tembusu virus plasmid vector and attenuated virus strain, and its application. Chinese Patent Application No.: CN202111308597.2. Filed on: November 5, 2021.
2. Shun Chen, Yu He, Anchun Cheng, Mingshu Wang. The method for an attenuated duck Tembusu virus vaccine preparation and its application. Chinese Patent Application No.: CN202110533162.1. Filed on: May 17, 2021.
3. Shun Chen, Yu He, Anchun Cheng, Mingshu Wang. The method for an attenuated duck Tembusu virus strain construction and its application. Chinese Patent Application No.: CN202110533132.0. Filed on: May 17, 2021.
4. Shun Chen, Yu He, Anchun Cheng, Mingshu Wang. A method for consturction of duck Tembusu reporter viurs and its product and application. Chinese Patent Application No.: CN201811402998.2. Filed on: November 23, 2018.
5. Renyong Jia, Jie Deng, Yanhong Liu, Anchun Cheng, Mingshu Wang, Shun Chen, Dekang Zhu, Mafeng Liu, Kunfeng Sun. A colloidal gold detection card for detecting duck Tembusu virus E protein antibody. Chinese Patent Application No.: CN201811094428.1. Filed on: September 19, 2018.
6. Shun Chen, Bowen Jiang, Wei Zhang, Anchun Cheng, Mingshu Wang. The expression method of truncated Tembusu virus nonstructural protein NS4B and its product and application. Chinese Patent Application No.: CN201810133024.2. Filed on: February 9, 2018.
7. Shun Chen, Tao Wang, Anchun Cheng, Mingshu Wang. Consturction and application of transient duck Tembusu viurs replicon expressing renilla luciferase. Chinese Patent Application No.: CN201711226242.2. Filed on: November 29, 2017.
8. Shun Chen, Yu He, Anchun Cheng, Mingshu Wang. Consturction and application of duck Tembusu reporter viurs expressing a renilla luciferase. Chinese Patent Application No.: CN201710702634.5. Filed on: August 16, 2017.
Contact Details
Renyong Jia, Ph.D, Professor
Institute of Preventive Veterinary Medicine
Sichuan Agricultural University
No. 211, Huimin Road, Chengdu, Sichuan 611130, China
Email:
jiary@sicau.edu.cn
Shun Chen, Ph.D, Professor
Institute of Preventive Veterinary Medicine
Sichuan Agricultural University
No. 211, Huimin Road, Chengdu, Sichuan 611130, China
Email:
shunchen@sicau.edu.cn
sophia_cs@163.com
Juan Huang, Ph.D, Lecturer
Institute of Preventive Veterinary Medicine
Sichuan Agricultural University
No. 211, Huimin Road, Chengdu, Sichuan 611130, China
Email:
huangjuan@sicau.edu.cn
huangjuan610@163.com
